Isolation and Purifecation of Riboflavin Binding Protein from Eagle Egg-yolk (aquila Hastate)
نویسنده
چکیده
Riboflavin binding protein was purified from the egg yolk of Aquila hastate (Eagle). The protein was purified using DEAE Sepharose ion exchange chromatography followed by gel filtration on Sephadex G-100. The purity of the protein was judged by cylindrical and slab SDSPAGE techniques. Comparison of the mobility of RfBP with that of the standard molecular weight marker protein revealed that the RfBP had a molecular weight close to 29 Kd. Interestingly the RfBPs from hen egg yolk and eagle egg yolk had the same molecular weights as revealed by the SDS PAGE.
منابع مشابه
ISOLATION AND PURIFICATION OF RIBOFLAVIN BINDING PROTIEN FROM COOT EGG-YOLK (Fulica atra)
Riboflavin-binding protein (RfBP) was isolated, purified and characterized from Coot (Fulica atra) egg. The RfBP was purified using DEAE-Sepharose ion exchange chromatography followed by gel filtration on Sephadex G-100. The purity of the proteins was judged by SDSPAGE. The protein migrated as a single band on SDS gel with a molecular weight of corresponding to nearly 29 Kd.
متن کاملIsolation, Purification and Characterization of Riboflavin Carrier Protein (RCP) from Emu Egg Yolk (Dromaius novaehollandia)
Riboflavin carrier (or binding) protein (RCP) was isolated from the Emu egg yolk (Dromaius novaehollandia) for the first time. In the present study an attempt has been made to isolate and purify the RCP in two steps, DEAE Sephadex A-50 ion exchange chromatography and the final purification was achieved on sephadex G-100.The purity of the protein was judged on cylindrical and slab gel electropho...
متن کاملComparison of Four Different Purification Methods for Isolation of Anti Echis carinatus Antivenom Antibodies from Immunized Chicken Egg Yolk
Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...
متن کاملSurgical treatment of bumblefoot in a captive golden eagle (Aquila chrysaetos)
The golden eagle is one of the world's largest living birds. Footpad dermatitis, also known as plantar pododermatitis or bumblefoot, is a condition characterized by lesions due to contact with unhealthy "perching" conditions, such as plastic perches, sharp-cornered perches on the ventral footpad of birds. A young female golden eagle (Aquila chrysaetos) in Fars province of Iran was presented to ...
متن کاملAvian riboflavin binding protein binds to lipoprotein receptors in association with vitellogenin.
Riboflavin binding protein (ribBP) is an essential component of chicken eggs; it supplies the oocyte (i.e. yolk) and egg white with sufficient amounts of the vitamin riboflavin to sustain embryonic development until hatching. There are three forms of ribBP in the laying hen; synthesized by the liver under the control of estrogen, it enters the serum (sribBP) and is delivered to yolk where it be...
متن کامل